Concatenate multiple text files of DNA sequences in Python or R? - python

I was wondering how to concatenate exon/DNA fasta files using Python or R.
Example files:
So far I really liked using R ape package for the cbind method, solely because of the fill.with.gaps=TRUE attribute. I really need gaps inserted when a species is missing an exon.
My code:
ex1 <- read.dna("exon1.txt", format="fasta")
ex2 <- read.dna("exon2.txt", format="fasta")
output <- cbind(ex1, ex2, fill.with.gaps=TRUE)
write.dna(output, "Output.txt", format="fasta")
Example:
exon1.txt
>sp1
AAAA
>sp2
CCCC
exon2.txt
>sp1
AGG-G
>sp2
CTGAT
>sp3
CTTTT
Output file:
>sp1
AAAAAGG-G
>sp2
CCCCCTGAT
>sp3
----CTTTT
So far I am having trouble trying to apply this technique when I have multiple exon files (trying to figure out a loop to open and execute the cbind method for all files ending with .fa in the directory), and sometimes not all files have exons that are all identical in length - hence DNAbin stops working.
So far I have:
file_list <- list.files(pattern=".fa")
myFunc <- function(x) {
for (file in file_list) {
x <- read.dna(file, format="fasta")
out <- cbind(x, fill.with.gaps=TRUE)
write.dna(out, "Output.txt", format="fasta")
}
}
However when I run this and I check my output text file, it misses many exons and I think that is because not all files have the same exon length... or my script is failing somewhere and I can't figure it out: (
Any ideas? I can also try Python.

If you prefer using Linux one liners you have
cat exon1.txt exon2.txt > outfile
if you want only the unique records from the outfile use
awk '/^>/{f=!d[$1];d[$1]=1}f' outfile > sorted_outfile

I just came out with this answer in Python 3:
def read_fasta(fasta): #Function that reads the files
output = {}
for line in fasta.split("\n"):
line = line.strip()
if not line:
continue
if line.startswith(">"):
active_sequence_name = line[1:]
if active_sequence_name not in output:
output[active_sequence_name] = []
continue
sequence = line
output[active_sequence_name].append(sequence)
return output
with open("exon1.txt", 'r') as file: # read exon1.txt
file1 = read_fasta(file.read())
with open("exon2.txt", 'r') as file: # read exon2.txt
file2 = read_fasta(file.read())
finaldict = {} #Concatenate the
for i in list(file1.keys()) + list(file2.keys()): #both files content
if i not in file1.keys():
file1[i] = ["-" * len(file2[i][0])]
if i not in file2.keys():
file2[i] = ["-" * len(file1[i][0])]
finaldict[i] = file1[i] + file2[i]
with open("output.txt", 'w') as file: # output that in file
for k, i in finaldict.items(): # named output.txt
file.write(">{}\n{}\n".format(k, "".join(i))) #proper formatting
It's pretty hard to comment and explain it completely, and it might not help you, but this is better than nothing :P
I used Ɓukasz Rogalski's code from answer to Reading a fasta file format into Python dict.

Related

How to solve problem decoding from wrong json format

everyone. Need help opening and reading the file.
Got this txt file - https://yadi.sk/i/1TH7_SYfLss0JQ
It is a dictionary
{"id0":"url0", "id1":"url1", ..., "idn":"urln"}
But it was written using json into txt file.
#This is how I dump the data into a txt
json.dump(after,open(os.path.join(os.getcwd(), 'before_log.txt'), 'a'))
So, the file structure is
{"id0":"url0", "id1":"url1", ..., "idn":"urln"}{"id2":"url2", "id3":"url3", ..., "id4":"url4"}{"id5":"url5", "id6":"url6", ..., "id7":"url7"}
And it is all a string....
I need to open it and check repeated ID, delete and save it again.
But getting - json.loads shows ValueError: Extra data
Tried these:
How to read line-delimited JSON from large file (line by line)
Python json.loads shows ValueError: Extra data
json.decoder.JSONDecodeError: Extra data: line 2 column 1 (char 190)
But still getting that error, just in different place.
Right now I got as far as:
with open('111111111.txt', 'r') as log:
before_log = log.read()
before_log = before_log.replace('}{',', ').split(', ')
mu_dic = []
for i in before_log:
mu_dic.append(i)
This eliminate the problem of several {}{}{} dictionaries/jsons in a row.
Maybe there is a better way to do this?
P.S. This is how the file is made:
json.dump(after,open(os.path.join(os.getcwd(), 'before_log.txt'), 'a'))
Your file size is 9,5M, so it'll took you a while to open it and debug it manually.
So, using head and tail tools (found normally in any Gnu/Linux distribution) you'll see that:
# You can use Python as well to read chunks from your file
# and see the nature of it and what it's causing a decode problem
# but i prefer head & tail because they're ready to be used :-D
$> head -c 217 111111111.txt
{"1933252590737725178": "https://instagram.fiev2-1.fna.fbcdn.net/vp/094927bbfd432db6101521c180221485/5CC0EBDD/t51.2885-15/e35/46950935_320097112159700_7380137222718265154_n.jpg?_nc_ht=instagram.fiev2-1.fna.fbcdn.net",
$> tail -c 219 111111111.txt
, "1752899319051523723": "https://instagram.fiev2-1.fna.fbcdn.net/vp/a3f28e0a82a8772c6c64d4b0f264496a/5CCB7236/t51.2885-15/e35/30084016_2051123655168027_7324093741436764160_n.jpg?_nc_ht=instagram.fiev2-1.fna.fbcdn.net"}
$> head -c 294879 111111111.txt | tail -c 12
net"}{"19332
So the first guess is that your file is a malformed series ofJSON data, and the best guess is to seperate }{ by a \n for further manipulations.
So, here is an example of how you can solve your problem using Python:
import json
input_file = '111111111.txt'
output_file = 'new_file.txt'
data = ''
with open(input_file, mode='r', encoding='utf8') as f_file:
# this with statement part can be replaced by
# using sed under your OS like this example:
# sed -i 's/}{/}\n{/g' 111111111.txt
data = f_file.read()
data = data.replace('}{', '}\n{')
seen, total_keys, to_write = set(), 0, {}
# split the lines of the in memory data
for elm in data.split('\n'):
# convert the line to a valid Python dict
converted = json.loads(elm)
# loop over the keys
for key, value in converted.items():
total_keys += 1
# if the key is not seen then add it for further manipulations
# else ignore it
if key not in seen:
seen.add(key)
to_write.update({key: value})
# write the dict's keys & values into a new file as a JSON format
with open(output_file, mode='a+', encoding='utf8') as out_file:
out_file.write(json.dumps(to_write) + '\n')
print(
'found duplicated key(s): {seen} from {total}'.format(
seen=total_keys - len(seen),
total=total_keys
)
)
Output:
found duplicated key(s): 43836 from 45367
And finally, the output file will be a valid JSON file and the duplicated keys will be removed with their values.
The basic difference between the file structure and actual json format is the missing commas and the lines are not enclosed within [. So the same can be achieved with the below code snippet
with open('json_file.txt') as f:
# Read complete file
a = (f.read())
# Convert into single line string
b = ''.join(a.splitlines())
# Add , after each object
b = b.replace("}", "},")
# Add opening and closing parentheses and ignore last comma added in prev step
b = '[' + b[:-1] + ']'
x = json.loads(b)

Python: Having trouble replacing lines from file

I'm trying to build a translator using deepl for subtitles but it isn't running perfectly. I managed to translate the subtitles and most of the part I'm having problems replacing the lines. I can see that the lines are translated because it prints them but it doesn't replace them. Whenever I run the program it is the same as the original file.
This is the code responsible for:
def translate(input, output, languagef, languaget):
file = open(input, 'r').read()
fileresp = open(output,'r+')
subs = list(srt.parse(file))
for sub in subs:
try:
linefromsub = sub.content
translationSentence = pydeepl.translate(linefromsub, languaget.upper(), languagef.upper())
print(str(sub.index) + ' ' + translationSentence)
for line in fileresp.readlines():
newline = fileresp.write(line.replace(linefromsub,translationSentence))
except IndexError:
print("Error parsing data from deepl")
This is the how the file looks:
1
00:00:02,470 --> 00:00:04,570
- Yes, I do.
- (laughs)
2
00:00:04,605 --> 00:00:07,906
My mom doesn't want
to babysit everyday
3
00:00:07,942 --> 00:00:09,274
or any day.
4
00:00:09,310 --> 00:00:11,977
But I need
my mom's help sometimes.
5
00:00:12,013 --> 00:00:14,046
She's just gonna
have to be grandma today.
Help will be appreaciated :)
Thanks.
You are opening fileresp with r+ mode. When you call readlines(), the file's position will be set to the end of the file. Subsequent calls to write() will then append to the file. If you want to overwrite the original contents as opposed to append, you should try this instead:
allLines = fileresp.readlines()
fileresp.seek(0) # Set position to the beginning
fileresp.truncate() # Delete the contents
for line in allLines:
fileresp.write(...)
Update
It's difficult to see what you're trying to accomplish with r+ mode here but it seems you have two separate input and output files. If that's the case consider:
def translate(input, output, languagef, languaget):
file = open(input, 'r').read()
fileresp = open(output, 'w') # Use w mode instead
subs = list(srt.parse(file))
for sub in subs:
try:
linefromsub = sub.content
translationSentence = pydeepl.translate(linefromsub, languaget.upper(), languagef.upper())
print(str(sub.index) + ' ' + translationSentence)
fileresp.write(translationSentence) # Write the translated sentence
except IndexError:
print("Error parsing data from deepl")

How to remove brackets and the contents inside from a file

I have a file named sample.txt which looks like below
ServiceProfile.SharediFCList[1].DefaultHandling=1
ServiceProfile.SharediFCList[1].ServiceInformation=
ServiceProfile.SharediFCList[1].IncludeRegisterRequest=n
ServiceProfile.SharediFCList[1].IncludeRegisterResponse=n
Here my requirement is to remove the brackets and the integer and enter os commands with that
ServiceProfile.SharediFCList.DefaultHandling=1
ServiceProfile.SharediFCList.ServiceInformation=
ServiceProfile.SharediFCList.IncludeRegisterRequest=n
ServiceProfile.SharediFCList.IncludeRegisterResponse=n
I am quite a newbie in Python. This is my first attempt. I have used these codes to remove the brackets:
#!/usr/bin/python
import re
import os
import sys
f = os.open("sample.txt", os.O_RDWR)
ret = os.read(f, 10000)
os.close(f)
print ret
var1 = re.sub("[\(\[].*?[\)\]]", "", ret)
print var1f = open("removed.cfg", "w+")
f.write(var1)
f.close()
After this using the file as input I want to form application specific commands which looks like this:
cmcli INS "DefaultHandling=1 ServiceInformation="
and the next set as
cmcli INS "IncludeRegisterRequest=n IncludeRegisterRequest=y"
so basically now I want the all the output to be bunched to a set of two for me to execute the commands on the operating system.
Is there any way that I could bunch them up as set of two?
Reading 10,000 bytes of text into a string is really not necessary when your file is line-oriented text, and isn't scalable either. And you need a very good reason to be using os.open() instead of open().
So, treat your data as the lines of text that it is, and every two lines, compose a single line of output.
from __future__ import print_function
import re
command = [None,None]
cmd_id = 1
bracket_re = re.compile(r".+\[\d\]\.(.+)")
# This doesn't just remove the brackets: what you actually seem to want is
# to pick out everything after [1]. and ignore the rest.
with open("removed_cfg","w") as outfile:
with open("sample.txt") as infile:
for line in infile:
m = bracket_re.match(line)
cmd_id = 1 - cmd_id # gives 0, 1, 0, 1
command[cmd_id] = m.group(1)
if cmd_id == 1: # we have a pair
output_line = """cmcli INS "{0} {1}" """.format(*command)
print (output_line, file=outfile)
This gives the output
cmcli INS "DefaultHandling=1 ServiceInformation="
cmcli INS "IncludeRegisterRequest=n IncludeRegisterResponse=n"
The second line doesn't correspond to your sample output. I don't know how the input IncludeRegisterResponse=n is supposed to become the output IncludeRegisterRequest=y. I assume that's a mistake.
Note that this code depends on your input data being precisely as you describe it and has no error checking whatsoever. So if the format of the input is in reality more variable than that, then you will need to add some validation.

grep in python properly

I am used to do scripting in bash, but I am also learning python.
So, as a way of learning, I am trying to modify my few old bash in python. As, say,I have a file, with line like:
TOTDOS= 0.38384E+02n_Ef= 0.81961E+02 Ebnd 0.86883E+01
to get the value of TOTDOS in bash, I just do:
grep "TOTDOS=" 630/out-Dy-eos2|head -c 19|tail -c 11
but by python, I am doing:
#!/usr/bin/python3
import re
import os.path
import sys
f1 = open("630/out-Dy-eos2", "r")
re1 = r'TOTDOS=\s*(.*)n_Ef=\s*(.*)\sEbnd'
for line in f1:
match1 = re.search(re1, line)
if match1:
TD = (match1.group(1))
f1.close()
print(TD)
Which is surely giving correct result, but seems to be much more then bash(not to mention problem with regex).
Question is, am I overworking in python, or missing something of it?
A python script that matches your bash line would be more like this:
with open('630/out-Dy-eos2', 'r') as f1:
for line in f1:
if "TOTDOS=" in line:
print line[8:19]
Looks a little bit better now.
[...] but seems to be much more than bash
Maybe (?) generators are the closest Python concept to the "pipe filtering" used in shell.
import itertools
#
# Simple generator to iterate through a file
# equivalent of line by line reading from an input file
def source(fname):
with open(fname,"r") as f:
for l in f:
yield l
src = source("630/out-Dy-eos2")
# First filter to keep only lines containing the required word
# equivalent to `grep -F`
filter1 = (l for l in src if "TOTDOS=" in l)
# Second filter to keep only line in the required range
# equivalent of `head -n ... | tail -n ...`
filter2 = itertools.islice(filter1, 10, 20,1)
# Finally output
output = "".join(filter2)
print(output)
Concerning your specific example, if you need it, you could use regexp in a generator:
re1 = r'TOTDOS=\s*(.*)n_Ef=\s*(.*)\sEbnd'
filter1 = (m.group(1) for m in (re.match(re1, l) for l in src) if m)
Those are only (some of the) basic building blocs available to you.

Bash or Python to go backwards?

I have a text file which a lot of random occurrences of the string #STRING_A, and I would be interested in writing a short script which removes only some of them. Particularly one that scans the file and once it finds a line which starts with this string like
#STRING_A
then checks if 3 lines backwards there is another occurrence of a line starting with the same string, like
#STRING_A
#STRING_A
and if it happens, to delete the occurrence 3 lines backward. I was thinking about bash, but I do not know how to "go backwards" with it. So I am sure that this is not possible with bash. I also thought about python, but then I should store all information in memory in order to go backwards and then, for long files it would be unfeasible.
What do you think? Is it possible to do it in bash or python?
Thanks
Funny that after all these hours nobody's yet given a solution to the problem as actually phrased (as #John Machin points out in a comment) -- remove just the leading marker (if followed by another such marker 3 lines down), not the whole line containing it. It's not hard, of course -- here's a tiny mod as needed of #truppo's fun solution, for example:
from itertools import izip, chain
f = "foo.txt"
for third, line in izip(chain(" ", open(f)), open(f)):
if third.startswith("#STRING_A") and line.startswith("#STRING_A"):
line = line[len("#STRING_A"):]
print line,
Of course, in real life, one would use an iterator.tee instead of reading the file twice, have this code in a function, not repeat the marker constant endlessly, &c;-).
Of course Python will work as well. Simply store the last three lines in an array and check if the first element in the array is the same as the value you are currently reading. Then delete the value and print out the current array. You would then move over your elements to make room for the new value and repeat. Of course when the array is filled you'd have to make sure to continue to move values out of the array and put in the newly read values, stopping to check each time to see if the first value in the array matches the value you are currently reading.
Here is a more fun solution, using two iterators with a three element offset :)
from itertools import izip, chain, tee
f1, f2 = tee(open("foo.txt"))
for third, line in izip(chain(" ", f1), f2):
if not (third.startswith("#STRING_A") and line.startswith("#STRING_A")):
print line,
Why shouldn't it possible in bash? You don't need to keep the whole file in memory, just the last three lines (if I understood correctly), and write what's appropriate to standard-out. Redirect that into a temporary file, check that everything worked as expected, and overwrite the source file with the temporary one.
Same goes for Python.
I'd provide a script of my own, but that wouldn't be tested. ;-)
As AlbertoPL said, store lines in a fifo for later use--don't "go backwards". For this I would definitely use python over bash+sed/awk/whatever.
I took a few moments to code this snippet up:
from collections import deque
line_fifo = deque()
for line in open("test"):
line_fifo.append(line)
if len(line_fifo) == 4:
# "look 3 lines backward"
if line_fifo[0] == line_fifo[-1] == "#STRING_A\n":
# get rid of that match
line_fifo.popleft()
else:
# print out the top of the fifo
print line_fifo.popleft(),
# don't forget to print out the fifo when the file ends
for line in line_fifo: print line,
This code will scan through the file, and remove lines starting with the marker. It only keeps only three lines in memory by default:
from collections import deque
def delete(fp, marker, gap=3):
"""Delete lines from *fp* if they with *marker* and are followed
by another line starting with *marker* *gap* lines after.
"""
buf = deque()
for line in fp:
if len(buf) < gap:
buf.append(line)
else:
old = buf.popleft()
if not (line.startswith(marker) and old.startswith(marker)):
yield old
buf.append(line)
for line in buf:
yield line
I've tested it with:
>>> from StringIO import StringIO
>>> fp = StringIO('''a
... b
... xxx 1
... c
... xxx 2
... d
... e
... xxx 3
... f
... g
... h
... xxx 4
... i''')
>>> print ''.join(delete(fp, 'xxx'))
a
b
xxx 1
c
d
e
xxx 3
f
g
h
xxx 4
i
This "answer" is for lyrae ... I'll amend my previous comment: if the needle is in the first 3 lines of the file, your script will either cause an IndexError or access a line that it shouldn't be accessing, sometimes with interesting side-effects.
Example of your script causing IndexError:
>>> lines = "#string line 0\nblah blah\n".splitlines(True)
>>> needle = "#string "
>>> for i,line in enumerate(lines):
... if line.startswith(needle) and lines[i-3].startswith(needle):
... lines[i-3] = lines[i-3].replace(needle, "")
...
Traceback (most recent call last):
File "<stdin>", line 2, in <module>
IndexError: list index out of range
and this example shows not only that the Earth is round but also why your "fix" to the "don't delete the whole line" problem should have used .replace(needle, "", 1) or [len(needle):] instead of .replace(needle, "")
>>> lines = "NEEDLE x NEEDLE y\nnoddle\nnuddle\n".splitlines(True)
>>> needle = "NEEDLE"
>>> # Expected result: no change to the file
... for i,line in enumerate(lines):
... if line.startswith(needle) and lines[i-3].startswith(needle):
... lines[i-3] = lines[i-3].replace(needle, "")
...
>>> print ''.join(lines)
x y <<<=== whoops!
noddle
nuddle
<<<=== still got unwanted newline in here
>>>
My awk-fu has never been that good... but the following may provide you what you're looking for in a bash-shell/shell-utility form:
sed `awk 'BEGIN{ORS=";"}
/#STRING_A/ {
if(LAST!="" && LAST+3 >= NR) print LAST "d"
LAST = NR
}' test_file` test_file
Basically... awk is producing a command for sed to strip certain lines. I'm sure there's a relatively easy way to make awk do all of the processing, but this does seem to work.
The bad part? It does read the test_file twice.
The good part? It is a bash/shell-utility implementation.
Edit: Alex Martelli points out that the sample file above might have confused me. (my above code deletes the whole line, rather than the #STRING_A flag only)
This is easily remedied by adjusting the command to sed:
sed `awk 'BEGIN{ORS=";"}
/#STRING_A/ {
if(LAST!="" && LAST+3 >= NR) print LAST "s/#STRING_A//"
LAST = NR
}' test_file` test_file
This may be what you're looking for?
lines = open('sample.txt').readlines()
needle = "#string "
for i,line in enumerate(lines):
if line.startswith(needle) and lines[i-3].startswith(needle):
lines[i-3] = lines[i-3].replace(needle, "")
print ''.join(lines)
this outputs:
string 0 extra text
string 1 extra text
string 2 extra text
string 3 extra text
--replaced -- 4 extra text
string 5 extra text
string 6 extra text
#string 7 extra text
string 8 extra text
string 9 extra text
string 10 extra text
In bash you can use sort -r filename and tail -n filename to read the file backwards.
$LINES=`tail -n filename | sort -r`
# now iterate through the lines and do your checking
I would consider using sed. gnu sed supports definition of line ranges. if sed would fail, then there is another beast - awk and I'm sure you can do it with awk.
O.K. I feel I should put my awk POC. I could not figure out to use sed addresses. I have not tried combination of awk+sed, but it seems to me it's overkill.
my awk script works as follows:
It reads lines and stores them into 3 line buffer
once desired pattern is found (/^data.*/ in my case), the 3-line buffer is looked up to check, whether desired pattern has been seen three lines ago
if pattern has been seen, then 3 lines are scratched
to be honest, I would probably go with python also, given that awk is really awkward.
the AWK code follows:
function max(a, b)
{
if (a > b)
return a;
else
return b;
}
BEGIN {
w = 0; #write index
r = 0; #read index
buf[0, 1, 2]; #buffer
}
END {
# flush buffer
# start at read index and print out up to w index
for (k = r % 3; k r - max(r - 3, 0); k--) {
#search in 3 line history buf
if (match(buf[k % 3], /^data.*/) != 0) {
# found -> remove lines from history
# by rewriting them -> adjust write index
w -= max(r, 3);
}
}
buf[w % 3] = $0;
w++;
}
/^.*/ {
# store line into buffer, if the history
# is full, print out the oldest one.
if (w > 2) {
print buf[r % 3];
r++;
buf[w % 3] = $0;
}
else {
buf[w] = $0;
}
w++;
}

Categories

Resources