Displaying a 32-bit image with NaN values (ImageJ) - python

I wrote a multilanguage 3-D image denoising ImageJ plugin that does some operations on an image and returns the denoised image as a 1-D array. The 1-D array contains NaN values (around the edges). The 1-D array is converted back into an image stack and displayed. It is simply black. I saved the image stack and opened it in ImageJ again. I moved my cursor over the image and saw the values change as they should. In some places (where I presume the neurone is) the pixels values were in the range of 1000-4000 . Yet, the whole image was simply pitch black. Here is a snippet of the code that converts the array into an image-stack at the end:
# Image-denoising routines written in C (this is where the nan values are introduced)
fimg = JNApackage.NativeCodeJNA.NativeCall(InputImgArray, medfiltArray, int(searchradius), int(patchradius), beta , int(x), int(y), int(z))
# Optimal Inverse Anscombe Transform (Some more operations in Jython)
fimg = InverseAnscombe.InvAnscombe(fimg)
InputImg.flush()
outputstack = ImageStack(x, y, z )
for i in xrange(0, z):
# Get the slice at index i and assign array elements corresponding to it.
outputstack.setPixels(fimg[int(i*x*y):int((i+1)*x*y)], i+1)
print 'Preparing denoised image for display '
outputImp = ImagePlus("Output Image", outputstack)
#print "OutputImage Stats:"
Stats = StackStatistics(outputImp)
print "mean:", Stats.mean, "minimum:", Stats.min, "maximum:", Stats.max
outputImp.show()
Any help as to what is going on?

The display range of your image might not be set correctly.
Try
outputImp.resetDisplayRange()
or
outputImp.setDisplayRange(Stats.min, Stats.max)
See the ImagePlus javadoc for more info.

Related

Luminance Correction (Prospective Correction)

When I was searching internet for an algorithm to correct luminance I came across this article about prospective correction and retrospective correction. I'm mostly interested in the prospective correction. Basically we take pictures of the scene with image in it(original one), and two other ,one bright and one dark, pictures where we only see the background of the original picture.
My problem is that I couldn't find any adaptation of these formulas in openCV or code example. I tried to use the formulas as they were in my code but this time I had a problem with data types. This happened when I tried to find C constant by applying operations on images.
This is how I implemented the formula in my code:
def calculate_C(im, im_b):
fx_mean = cv.mean(im)
fx_over_bx = np.divide(im,im_b)
mean_fx_bx = cv.mean(fx_over_bx)
c = np.divide(fx_mean, mean_fx_bx)
return c
#Basic image reading and resizing
# Original image
img = cv.imread(image_path)
img = cv.resize(img, (1000,750))
# Bright image
b_img = cv.imread(bright_image_path)
b_img = cv.resize(b_img, (1000,750))
# Calculating C constant from the formula
c_constant = calculate_C(img, b_img)
# Because I have only the bright image I am using second formula from the article
img = np.multiply(np.divide(img,b_img), c_constant)
When I try to run this code I get the error:
img = np.multiply(np.divide(img,b_img), c_constant)
ValueError: operands could not be broadcast together with shapes (750,1000,3) (4,)
So, is there anything I can do to fix my code? or is there any hints that you can share with me to handle luminance correction with this method or better methods?
You are using cv2.mean function which returns array with shape (4,) - mean value for each channel. You may need to ignore last channel and correctly broadcast it to numpy.
Or you could use numpy for calculations instead of opencv.
I just take example images from provided article.
grain.png:
grain_background.png:
Complete example:
import cv2
import numpy as np
from numpy.ma import divide, mean
f = cv2.imread("grain.png")
b = cv2.imread("grain_background.png")
f = f.astype(np.float32)
b = b.astype(np.float32)
C = mean(f) / divide(f, b).mean()
g = divide(f, b) * C
g = g.astype(np.uint8)
cv2.imwrite("grain_out.png", g)
Your need to use masked divide operation because ordinary operation could lead to division by zero => nan values.
Resulting image (output.png):

saving multiple 3D array of size (22,6,2840) as images

I am generating multiple 3D numpy array of size (22,6,2840),each array containing 22 array of size(6,2840).Now I want to save this array (22,6,2840) as images. I don't know if I can do that. I tried to do this using plt.savefig but it didn't work. I am trying for more than 2 weeks to find how I can do it.
Any help would be appreciated.
signals=np.zeros((22,6,2840))
t=0
movement=int(S*256)
if(S==0):
movement=_SIZE_WINDOW_SPECTOGRAM
while data.shape[1]-(t*movement+_SIZE_WINDOW_SPECTOGRAM) > 0:
for i in range(0, 22):
start = t*movement
stop = start+_SIZE_WINDOW_SPECTOGRAM
signals[i,:]=wavelet(data[i,start:stop])
if(signalsBlock is None):
signalsBlock=np.array([signals])
else:
signalsBlock=np.append(signalsBlock, [signals], axis=0)
nSpectogram=nSpectogram+1
if(signalsBlock.shape[0]==50):
saveSignalsOnDisk(signalsBlock, nSpectogram)
signalsBlock=None
t = t+1
try using the PyPNG library. You will have to reshape your array to a 2-D format and then write it as a png. The link to the library is here
image_2d = numpy.reshape(image_3d, (-1, column_count * plane_count))
pngWriter.write(out, image_2d)
Also, one more method by using PIL Image is provided here. However, that works with mostly RGB style 3 channel images.

Creating an ASCII art world map

I'd like to render an ASCII art world map given this GeoJSON file.
My basic approach is to load the GeoJSON into Shapely, transform the points using pyproj to Mercator, and then do a hit test on the geometries for each character of my ASCII art grid.
It looks (edit: mostly) OK when centered one the prime meridian:
But centered on New York City (lon_0=-74), and it suddenly goes haywire:
I'm fairly sure I'm doing something wrong with the projections here. (And it would probably be more efficient to transform the ASCII map coordinates to lat/lon than transform the whole geometry, but I am not sure how.)
import functools
import json
import shutil
import sys
import pyproj
import shapely.geometry
import shapely.ops
# Load the map
with open('world-countries.json') as f:
countries = []
for feature in json.load(f)['features']:
# buffer(0) is a trick for fixing polygons with overlapping coordinates
country = shapely.geometry.shape(feature['geometry']).buffer(0)
countries.append(country)
mapgeom = shapely.geometry.MultiPolygon(countries)
# Apply a projection
tform = functools.partial(
pyproj.transform,
pyproj.Proj(proj='longlat'), # input: WGS84
pyproj.Proj(proj='webmerc', lon_0=0), # output: Web Mercator
)
mapgeom = shapely.ops.transform(tform, mapgeom)
# Convert to ASCII art
minx, miny, maxx, maxy = mapgeom.bounds
srcw = maxx - minx
srch = maxy - miny
dstw, dsth = shutil.get_terminal_size((80, 20))
for y in range(dsth):
for x in range(dstw):
pt = shapely.geometry.Point(
(srcw*x/dstw) + minx,
(srch*(dsth-y-1)/dsth) + miny # flip vertically
)
if any(country.contains(pt) for country in mapgeom):
sys.stdout.write('*')
else:
sys.stdout.write(' ')
sys.stdout.write('\n')
I made edit at the bottom, discovering new problem (why there is no Canada and unreliability of Shapely and Pyproj)
Even though its not exactly solving the problem, I believe this attitude has more potential than using pyproc and Shapely and in future, if you will do more Ascii art, will give you more possibilites and flexibility. Firstly I will write pros and cons.
PS: Initialy I wanted to find problem in your code, but I had problems with running it, because pyproj was returning me some error.
PROS
1) I was able to extract all points (Canada is really missing) and rotate image
2) The processing is very fast and therefore you can create Animated Ascii art.
3) Printing is done all at once without need to loop
CONS (known Issues, solvable)
1) This attitude is definetly not translating geo-coordinates correctly - too plane, it should look more spherical
2) I didnt take time to try to find out solution to filling the borders, so only borders has '*'. Therefore this attitude needs to find algorithm to fill the countries. I think it shouldnt be problem since the JSON file contains countries separated
3) You need 2 extra libs beside numpy - opencv(you can use PIL instead) and Colorama, because my example is animated and I needed to 'clean' terminal by moving cursor to (0,0) instead of using os.system('cls')
4) I made it run only in python 3. In python 2 it works too but I am getting error with sys.stdout.buffer
Change font size on terminal to lowest point so the the printed chars fit in terminal. Smaller the font, better resolution
The animation should look like the map is 'rotating'
I used little bit of your code to extract the data. Steps are in the commentaries
import json
import sys
import numpy as np
import colorama
import sys
import time
import cv2
#understand terminal_size as how many letters in X axis and how many in Y axis. Sorry not good name
if len(sys.argv)>1:
terminal_size = (int(sys.argv[1]),int(sys.argv[2]))
else:
terminal_size=(230,175)
with open('world-countries.json') as f:
countries = []
minimal = 0 # This can be dangerous. Expecting negative values
maximal = 0 # Expecting bigger values than 0
for feature in json.load(f)['features']: # getting data - I pretend here, that geo coordinates are actually indexes of my numpy array
indexes = np.int16(np.array(feature['geometry']['coordinates'][0])*2)
if indexes.min()<minimal:
minimal = indexes.min()
if indexes.max()>maximal:
maximal = indexes.max()
countries.append(indexes)
countries = (np.array(countries)+np.abs(minimal)) # Transform geo-coordinates to image coordinates
correction = np.abs(minimal) # because geo-coordinates has negative values, I need to move it to 0 - xaxis
colorama.init()
def move_cursor(x,y):
print ("\x1b[{};{}H".format(y+1,x+1))
move = 0 # 'rotate' the globe
for i in range(1000):
image = np.zeros(shape=[maximal+correction+1,maximal+correction+1]) #creating clean image
move -=1 # you need to rotate with negative values
# because negative one are by numpy understood. Positive one will end up with error
for i in countries: # VERY STRANGE,because parsing the json, some countries has different JSON structure
if len(i.shape)==2:
image[i[:,1],i[:,0]+move]=255 # indexes that once were geocoordinates now serves to position the countries in the image
if len(i.shape)==3:
image[i[0][:,1],i[0][:,0]+move]=255
cut = np.where(image==255) # Bounding box
if move == -1: # creating here bounding box - removing empty edges - from sides and top and bottom - we need space. This needs to be done only once
max_x,min_x = cut[0].max(),cut[0].min()
max_y,min_y = cut[1].max(),cut[1].min()
new_image = image[min_x:max_x,min_y:max_y] # the bounding box
new_image= new_image[::-1] # reverse, because map is upside down
new_image = cv2.resize(new_image,terminal_size) # resize so it fits inside terminal
ascii = np.chararray(shape = new_image.shape).astype('|S4') #create container for asci image
ascii[:,:]='' #chararray contains some random letters - dunno why... cleaning it
ascii[:,-1]='\n' #because I pring everything all at once, I am creating new lines at the end of the image
new_image[:,-1]=0 # at the end of the image can be country borders which would overwrite '\n' created one step above
ascii[np.where(new_image>0)]='*' # transforming image array to chararray. Better to say, anything that has pixel value higher than 0 will be star in chararray mask
move_cursor(0,0) # 'cleaning' the terminal for new animation
sys.stdout.buffer.write(ascii) # print into terminal
time.sleep(0.025) # FPS
Maybe it would be good to explain what is the main algorithm in the code. I like to use numpy whereever I can. The whole thing is that I pretend that coordinates in the image, or whatever it may be (in your case geo-coordinates) are matrix indexes. I have then 2 Matrixes - Real Image and Charray as Mask. I then take indexes of interesting pixels in Real image and for the same indexes in Charray Mask I assign any letter I want. Thanks to this, the whole algorithm doesnt need a single loop.
About Future posibilities
Imagine you will also have information about terrain(altitude). Let say you somehow create grayscale image of world map where gray shades expresses altitude. Such grayscale image would have shape x,y. You will prepare 3Dmatrix with shape = [x,y,256]. For each layer out of 256 in the 3D matrix, you assign one letter ' ....;;;;### and so on' that will express shade.
When you have this prepared, you can take your grayscale image where any pixel will actually have 3 coordinates: x,y and shade value. So you will have 3 arrays of indexes out of your grascale map image -> x,y,shade. Your new charray will simply be extraction of your 3Dmatrix with layer letters, because:
#Preparation phase
x,y = grayscale.shape
3Dmatrix = np.chararray(shape = [x,y,256])
table = ' ......;;;;;;;###### ...'
for i in range(256):
3Dmatrix[:,:,i] = table[i]
x_indexes = np.arange(x*y)
y_indexes = np.arange(x*y)
chararray_image = np.chararray(shape=[x,y])
# Ready to print
...
shades = grayscale.reshape(x*y)
chararray_image[:,:] = 3Dmatrix[(x_indexes ,y_indexes ,shades)].reshape(x,y)
Because there is no loop in this process and you can print chararray all at once, you can actually print movie into terminal with huge FPS
For example if you have footage of rotating earth, you can make something like this - (250*70 letters), render time 0.03658s
You can ofcourse take it into extreme and make super-resolution in your terminal, but resulting FPS is not that good: 0.23157s, that is approximately 4-5 FPS. Interesting to note is, that this attitude FPS is enourmous, but terminal simply cannot handle printing, so this low FPS is due to limitations of terminal and not of calculation as calculation of this high resolution took 0.00693s, that is 144 FPS.
BIG EDIT - contradicting some of above statements
I accidentaly opened raw json file and find out, there is CANADA and RUSSIA with full correct coordinates. I made mistake to rely on the fact that we both didnt have canada in the result, so I expected my code is ok. Inside JSON, the data has different NOT-UNIFIED structure. Russia and Canada has 'Multipolygon', so you need to iterate over it.
What does it mean? Dont rely on Shapely and pyproj. Obviously they cant extract some countries and if they cant do it reliably, you cant expect them to do anything more complicated.
After modifying the code, everything is allright
CODE: This is how to load the file correctly
...
with open('world-countries.json') as f:
countries = []
minimal = 0
maximal = 0
for feature in json.load(f)['features']: # getting data - I pretend here, that geo coordinates are actually indexes of my numpy array
for k in range((len(feature['geometry']['coordinates']))):
indexes = np.int64(np.array(feature['geometry']['coordinates'][k]))
if indexes.min()<minimal:
minimal = indexes.min()
if indexes.max()>maximal:
maximal = indexes.max()
countries.append(indexes)
...

Load sequence of PNGs into vtkImageData for 3D volume render using python

I have a sequence of about 100 PNG files containing 512x512 pre-segmented CAT scan data. I want to use vtk on Python to create a 3D model using marching cubes algorithm. The part that I don't know how to do is to load the sequence of PNG files and convert them to a single vtk pixel data object suitable for sending to the vtkDiscreteMarchingCubes algorithm.
I also think that I need to convert the pixel values of the PNG data because right now the data is in the alpha channel, so this needs to be converted into scalar data with values of zero and 1.
use vtkPNGreader and load in individual slices and then populate a vtkImageData which you can define the dimensions as and for each z-slice or image fill the image data form the output of the reader into your vtkImageData.
Rough pseudocode - not checked for bugs :)
import vtk
from vtk.util import numpy_support
pngfiles = glob.glob('*.png')
png_reader = vtk.vtkPNGReader()
png_reader.SetFileName(pngfiles[0])
x,y = png_reader.GetOutput().GetDimensions()
data_3d = np.zeros([x,y,len(pngfiles)])
for i,p in enumerate(png):
png_reader.SetFileName(pngfiles[0])
png_reader.Update()
img_data = png_reader.GetOutput()
data_3D[:,:,i] = numpy_support.vtk_to_numpy(img_data)
#save your 3D numpy array out.
data_3Dvtk = numpy_support.numpy_to_vtk(data_3D)
Just in case anyone stumbles on here looking for another way to do this only using vtk, you can use vtkImageAppend class.
def ReadImages(files):
reader = vtk.vtkPNGReader()
image3D = vtk.vtkImageAppend()
image3D.SetAppendAxis(2)
for f in files:
reader.SetFileName(f)
reader.Update()
t_img = vtk.vtkImageData()
t_img.DeepCopy(reader.GetOutput())
image3D.AddInputData(t_img)
image3D.Update()
return image3D.GetOutput()
for converting the data you can take a look at what the output of t_img.GetPointData().GetArray('PNGImage') gives and see if it is the expected value.

NumPy, PIL adding an image

I'm trying to add two images together using NumPy and PIL. The way I would do this in MATLAB would be something like:
>> M1 = imread('_1.jpg');
>> M2 = imread('_2.jpg');
>> resM = M1 + M2;
>> imwrite(resM, 'res.jpg');
I get something like this:
alt text http://www.deadlink.cc/matlab.jpg
Using a compositing program and adding the images the MATLAB result seems to be right.
In Python I'm trying to do the same thing like this:
from PIL import Image
from numpy import *
im1 = Image.open('/Users/rem7/Desktop/_1.jpg')
im2 = Image.open('/Users/rem7/Desktop/_2.jpg')
im1arr = asarray(im1)
im2arr = asarray(im2)
addition = im1arr + im2arr
resultImage = Image.fromarray(addition)
resultImage.save('/Users/rem7/Desktop/a.jpg')
and I get something like this:
alt text http://www.deadlink.cc/python.jpg
Why am I getting all those funky colors? I also tried using ImageMath.eval("a+b", a=im1, b=im2), but I get an error about RGB unsupported.
I also saw that there is an Image.blend() but that requires an alpha.
What's the best way to achieve what I'm looking for?
Source Images (images have been removed):
alt text http://www.deadlink.cc/_1.jpg
alt text http://www.deadlink.cc/_2.jpg
Humm, OK, well I added the source images using the add image icon and they show up when I'm editing the post, but for some reason the images don't show up in the post.
(images have been removed) 2013 05 09
As everyone suggested already, the weird colors you're observing are overflow. And as you point out in the comment of schnaader's answer you still get overflow if you add your images like this:
addition=(im1arr+im2arr)/2
The reason for this overflow is that your NumPy arrays (im1arr im2arr) are of the uint8 type (i.e. 8-bit). This means each element of the array can only hold values up to 255, so when your sum exceeds 255, it loops back around 0:
>>>array([255,10,100],dtype='uint8') + array([1,10,160],dtype='uint8')
array([ 0, 20, 4], dtype=uint8)
To avoid overflow, your arrays should be able to contain values beyond 255. You need to convert them to floats for instance, perform the blending operation and convert the result back to uint8:
im1arrF = im1arr.astype('float')
im2arrF = im2arr.astype('float')
additionF = (im1arrF+im2arrF)/2
addition = additionF.astype('uint8')
You should not do this:
addition = im1arr/2 + im2arr/2
as you lose information, by squashing the dynamic of the image (you effectively make the images 7-bit) before you perform the blending information.
MATLAB note: the reason you don't see this problem in MATLAB, is probably because MATLAB takes care of the overflow implicitly in one of its functions.
Using PIL's blend() with an alpha value of 0.5 would be equivalent to (im1arr + im2arr)/2. Blend does not require that the images have alpha layers.
Try this:
from PIL import Image
im1 = Image.open('/Users/rem7/Desktop/_1.jpg')
im2 = Image.open('/Users/rem7/Desktop/_2.jpg')
Image.blend(im1,im2,0.5).save('/Users/rem7/Desktop/a.jpg')
To clamp numpy array values:
>>> c = a + b
>>> c[c > 256] = 256
It seems the code you posted just sums up the values and values bigger than 256 are overflowing. You want something like "(a + b) / 2" or "min(a + b, 256)". The latter seems to be the way that your Matlab example does it.
Your sample images are not showing up form me so I am going to do a bit of guessing.
I can't remember exactly how the numpy to pil conversion works but there are two likely cases. I am 95% sure it is 1 but am giving 2 just in case I am wrong.
1) 1 im1Arr is a MxN array of integers (ARGB) and when you add im1arr and im2arr together you are overflowing from one channel into the next if the components b1+b2>255. I am guessing matlab represents their images as MxNx3 arrays so each color channel is separate. You can solve this by splitting the PIL image channels and then making numpy arrays
2) 1 im1Arr is a MxNx3 array of bytes and when you add im1arr and im2arr together you are wrapping the component around.
You are also going to have to rescale the range back to between 0-255 before displaying. Your choices are divide by 2, scale by 255/array.max() or do a clip. I don't know what matlab does

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