Counting cells with OpenCV - python

I'd like to believe I'm close to being able to count cells, but I also know I'm missing something.
I have this image
image = cv2.imread('/cells.png',
cv2.IMREAD_GRAYSCALE)
image = cv2.resize(image, (1000, 600))
th, threshedImg = cv2.threshold(image, 30, 255,cv2.THRESH_TOZERO_INV)
img_blur = cv2.GaussianBlur(threshedImg, (3,3), 0)
sobelxy = cv2.Sobel(src=img_blur,
ddepth=cv2.CV_64F,
dx=1, dy=1, ksize=5)
edges = cv2.Canny(image=img_blur,
threshold1=20,
threshold2=65)
cv2.imshow('thresh', threshedImg)
cv2.imshow('Canny Edge Detection', edges)
cv2.waitKey(0)
Edge output:
Threshold output:
The threshold image captures the cells pretty good from what I can tell, and the Canny does a pretty good job for getting the edges. Ive tried making use of contours, but I was unable to produce any good results.
Any help or ideas on how to improve would be appreciated. Thanks!

I count 286 blobs. That depends on some tweakables because some blobs that could be counted separately are really close together.
Approach:
Your input has some grayish background in the bottom right. To compensate, I estimate the background using a large median blur, kernel size ~100, and subtract that (saturating math).
Next, I blur the entire thing to suppress noise sufficiently that each blob is smooth (just one local maximum on it, no camel humps or worse)
Then I use image == cv.dilate(image, iterations=15) to calculate a mask of local extrema.
Then I combine that with a mask of peaks which is simply image > threshold.
I & (and) both masks together.
morphological close/dilate operation to merge some peaks that occur due to numerics (anything the smoothing hasn't smoothed enough that the dilate and equality only see one peak)
Then I use connectedComponentsWithStats to find all those blobs and their centroids.
My opinion on other approaches:
Canny, makes absolute no sense at all. Will leave you in a worse place.
Color Space transformation... pointless because your input is basically monochrome and I treat it as such.

Related

How can I smoothly detect the welding joint using OpenCV-Python?

I have tried to detect the welding joint (bead) using the codes attached below in the last part of this question. I aim to draw a contour on the joint as shown in the third image but my results are poor and don't look similar to the expected results. here is the summary of what I did but the codes are well clear:
1. Reading the image .jpg format
2. Image blurring
3. Thresholding
4. Morphological operations
5. Creating mask
6. Finding contour
But the results are not promising, how can I get out from here
img_new = cv2.cvtColor(img,cv2.COLOR_BGR2GRAY)
blur_image = cv2.bilateralFilter(img_new,5,21,21)
thresh = cv2.adaptiveThreshold(blur_image,255,
cv2.ADAPTIVE_THRESH_GAUSSIAN_C,
cv2.THRESH_BINARY_INV,15,3)
kernel = np.ones((5,5),dtype='uint8')
thresh_dilated = cv2.dilate(thresh, kernel, iterations = 1)
num_labels, labels, stats, centroids = cv2.connectedComponentsWithStats(thresh_dilated, connectivity=4)
sizes = stats[1:,-1]
min_size = 5000
num_labels = num_labels -1
img2 = np.zeros((labels.shape))
for i in range(0,num_labels):
if sizes[i]>=min_size:
img2[labels==i+1]=255
closing = cv2.morphologyEx(img2,cv2.MORPH_CLOSE,kernel)
opening = cv2.morphologyEx(closing,cv2.MORPH_OPEN,kernel)
result = opening.copy()
new_result = result.astype(dtype=np.uint8)
black = np.full((new_result.shape[0],new_result.shape[1],3),(0,0,0),np.uint8)
black1 = cv2.ellipse(black,(700,750),(300,140),0,0,360,(255,255,255),-1)
grayscale = cv2.cvtColor(black1,cv2.COLOR_BGR2GRAY)
ret,b_mask = cv2.threshold(grayscale,127,255,0)
img_result = cv2.bitwise_and(new_result,b_mask)
contours, hierarchy = cv2.findContours(img_result, cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE)
cv2.drawContours(img, contours, -1, (0,255,0), 2)
cv2.imshow('result',img)
cv2.waitKey(0)```
Read any papers?
Is this for automatic processing of many welding joints on an assembly line? Does this have to work for many other similiar images? It does not make sense to fine tune an algorithm for a single picture in that case.
If yes, than you need to use more images to create the algorithm, maybe use a different light setup, maybe images taken with an IR camera right after the welding to get a "hot" mask. Or use light from left and right sight separately to combine two images for a single mark.
Another thing that would be very helpful is to get a "before" image from the parts. without the welding joint. In that case it could get easy. You would just create the difference between the images, do some filtering to remove the welding beads and the reddish layer.
Edit 1:
Another thing I forgot to mention is please look at the RGB layers separately. This is something that you should always try early on. Often there is something useful to see, e.g. in your case it could be that the blue layer might be interesting. Please add the layers to your question.

OpenCV Detect scratches on fruits

For a little experiment in Python I'm doing I want to find small scratches on fruits. The scratches are very small and hard to detect by human eye.
I'm using a high resolution camera for that experiment.
Here is the defect I want to detect:
Original Image:
This is my result with very few lines of code:
So I found the contours of my fruit. How can I proceed to finding the scratch? The RGB Value is similar to other parts of the fruit. So how can I differentiate between A scratch, and a part of the fruit?
My code:
# Imports
import numpy as np
import cv2
import time
# Read Image & Convert
img = cv2.imread('IMG_0441.jpg')
result = cv2.cvtColor(img, cv2.COLOR_BGR2HSV)
# Filtering
lower = np.array([1,60,50])
upper = np.array([255,255,255])
result = cv2.inRange(result, lower, upper)
kernel = cv2.getStructuringElement(cv2.MORPH_ELLIPSE,(9,9))
result = cv2.dilate(result,kernel)
# Contours
im2, contours, hierarchy = cv2.findContours(result.copy(), cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE)
result = cv2.cvtColor(result, cv2.COLOR_GRAY2BGR)
if len(contours) != 0:
for (i, c) in enumerate(contours):
area = cv2.contourArea(c)
if area > 100000:
print(area)
cv2.drawContours(img, c, -1, (255,255,0), 12)
x,y,w,h = cv2.boundingRect(c)
cv2.rectangle(img,(x,y),(x+w,y+h),(0,255,0),12)
# Stack results
result = np.vstack((result, img))
resultOrig = result.copy()
# Save image to file before resizing
cv2.imwrite(str(time.time())+'_0_result.jpg',resultOrig)
# Resize
max_dimension = float(max(result.shape))
scale = 900/max_dimension
result = cv2.resize(result, None, fx=scale, fy=scale)
# Show results
cv2.imshow('res',result)
cv2.waitKey(0)
cv2.destroyAllWindows()
I changed your image to HSL colour space.
I can't see the scratch in the L channel, so the greyscale approach suggested earlier is going to be difficult.
But the scratch is quite noticeable in the hue plane.
You could use an edge detector to find the blemish in the hue channel. Here I use a difference of gaussians detector (with sizes 20 and 4).
personal guess is to use some algorithm to detect the grayscale change. The grayscale variation around the scratch should be bigger than the variation in other area. Sobel and Scharr Derivatives could be an option. This is a link to python-openCV about image gradient. You can first crop out the fruit with coutour application
If you really want to use conventional computer vision techniques, you should start with edges that can be detected on the fruit. Some of the edges are caused by the bumps on the fruit, so you have to look at various features of the area around the edges to find the difference between scratches and bumps. After you look at about a hundred scratches, you should be able to come up with some rules.
But this process is going to be very tiring, and my guess is you will not have much luck. A better way to approach this problem is to train a deep neural network by manually annotating scratches on about 100 images, and letting the network find out by itself how to distinguish scratches from the rest of the fruit.
If you are a beginner to these stuff, search for PyImageSearch and LearnOpenCV. Both are very resourceful sites where you can learn quickly.

How to detect rectangular items in image with Python

I have found a plethora of questions regarding finding "things" in images using openCV, et al. in Python but so far I have been unable to piece them together for a reliable solution to my problem.
I am attempting to use computer vision to help count tiny surface mount electronics parts. The idea is for me to dump parts onto a solid color piece of paper, snap a picture, and have the software tell me how many items are in it.
The "things" differ from one picture to the next but will always be identical in any one image. I seem to be able to manually tune the parameters for things like hue/saturation for a particular part but it tends to require tweaking every time I change to a new part.
My current, semi-functioning code is posted below:
import imutils
import numpy
import cv2
import sys
def part_area(contours, round=10):
"""Finds the mode of the contour area. The idea is that most of the parts in an image will be separated and that
finding the most common area in the list of areas should provide a reasonable value to approximate by. The areas
are rounded to the nearest multiple of 200 to reduce the list of options."""
# Start with a list of all of the areas for the provided contours.
areas = [cv2.contourArea(contour) for contour in contours]
# Determine a threshold for the minimum amount of area as 1% of the overall range.
threshold = (max(areas) - min(areas)) / 100
# Trim the list of areas down to only those that exceed the threshold.
thresholded = [area for area in areas if area > threshold]
# Round the areas to the nearest value set by the round argument.
rounded = [int((area + (round / 2)) / round) * round for area in thresholded]
# Remove any areas that rounded down to zero.
cleaned = [area for area in rounded if area != 0]
# Count the areas with the same values.
counts = {}
for area in cleaned:
if area not in counts:
counts[area] = 0
counts[area] += 1
# Reduce the areas down to only those that are in groups of three or more with the same area.
above = []
for area, count in counts.iteritems():
if count > 2:
for _ in range(count):
above.append(area)
# Take the mean of the areas as the average part size.
average = sum(above) / len(above)
return average
def find_hue_mode(hsv):
"""Given an HSV image as an input, compute the mode of the list of hue values to find the most common hue in the
image. This is used to determine the center for the background color filter."""
pixels = {}
for row in hsv:
for pixel in row:
hue = pixel[0]
if hue not in pixels:
pixels[hue] = 0
pixels[hue] += 1
counts = sorted(pixels.keys(), key=lambda key: pixels[key], reverse=True)
return counts[0]
if __name__ == "__main__":
# load the image and resize it to a smaller factor so that the shapes can be approximated better
image = cv2.imread(sys.argv[1])
# define range of blue color in HSV
hsv = cv2.cvtColor(image, cv2.COLOR_BGR2HSV)
center = find_hue_mode(hsv)
print 'Center Hue:', center
lower = numpy.array([center - 10, 50, 50])
upper = numpy.array([center + 10, 255, 255])
# Threshold the HSV image to get only blue colors
mask = cv2.inRange(hsv, lower, upper)
inverted = cv2.bitwise_not(mask)
blurred = cv2.GaussianBlur(inverted, (5, 5), 0)
edged = cv2.Canny(blurred, 50, 100)
dilated = cv2.dilate(edged, None, iterations=1)
eroded = cv2.erode(dilated, None, iterations=1)
# find contours in the thresholded image and initialize the shape detector
contours = cv2.findContours(eroded.copy(), cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE)
contours = contours[0] if imutils.is_cv2() else contours[1]
# Compute the area for a single part to use when setting the threshold and calculating the number of parts within
# a contour area.
part_area = part_area(contours)
# The threshold for a part's area - can't be too much smaller than the part itself.
threshold = part_area * 0.5
part_count = 0
for contour in contours:
if cv2.contourArea(contour) < threshold:
continue
# Sometimes parts are close enough together that they become one in the image. To battle this, the total area
# of the contour is divided by the area of a part (derived earlier).
part_count += int((cv2.contourArea(contour) / part_area) + 0.1) # this 0.1 "rounds up" slightly and was determined empirically
# Draw an approximate contour around each detected part to give the user an idea of what the tool has computed.
epsilon = 0.1 * cv2.arcLength(contour, True)
approx = cv2.approxPolyDP(contour, epsilon, True)
cv2.drawContours(image, [approx], -1, (0, 255, 0), 2)
# Print the part count and show off the processed image.
print 'Part Count:', part_count
cv2.imshow("Image", image)
cv2.waitKey(0)
Here's an example of the type of input image I am using:
or this:
And I'm currently getting results like this:
The results clearly show that the script is having trouble identifying some parts and it's true Achilles heel seems to be when parts touch one another.
So my question/challenge is, what can I do to improve the reliability of this script?
The script is to be integrated into an existing Python tool so I am searching for a solution using Python. The solution does not need to be pure Python as I am willing to install whatever 3rd party libraries might be needed.
If the objects are all of similar types, you might have more success isolating a single example in the image and then using feature matching to detect them.
A full solution would be out of scope for Stack Overflow, but my suggestion for progress would be to first somehow find one or more "correct" examples using your current rectangle retrieval method. You could probably look for all your samples that are of the expected size, or that are accurate rectangles.
Once you have isolated a few positive examples, use some feature matching techniques to find the others. There is a lot of reading up you probably need to do on it but that is a potential solution.
A general summary is that you use your positive examples to find "features" of the object you want to detect. These "features" are generally things like corners or changes in gradient. OpenCV contains many methods you can use.
Once you have the features, there are several algorithms in OpenCV you can look at that will search the image for all matching features. You’ll want one that is rotation invariant (can detect the same features arranged in different rotation), but you probably don’t need scale invariance (can detect the same features at multiple scales).
My one concern with this method is that the items you are searching for in your images are quite small. It might be difficult to find good, consistent features to match on.
You're tackling a 2D object recognition problem, for which there are many possible approaches. You've gone about it using background/foreground segmentation, which is ok as you have control on the scene (laying down the background paper sheet). However this will always have fundamental limitations when the objects touch. A simple solution to your problem can be this:
1) You assume that touching objects are rare events (which is a fine assumption in your problem). Therefore you can compute the areas for each segmented region, and compute the median of these, which will give a robust estimate for the object's area. Let's call this robust estimate A (in squared pixels). This will be fine if fewer than 50% of regions correspond to touching objects.
2) You then proceed to measure the number of objects in each segmented region. Let Ai be the area of the ith region. You then compute the number of objects in each region by Ni=round(Ai/A). You then sum Ni to give you the total number of objects.
This approach will be fine as long as the following conditions are met:
A) The touching objects do not significantly overlap
B) You do not have objects lying on their sides. If you do you might be able to deal with this using two area estimates (side and flat). Better to eliminate this scenario if you can for simplicity.
C) The objects are all roughly the same distance to the camera. If this is not the case then the areas of the objects (in pixels) cannot be modelled well by a single value.
D) There are not partially visible objects at the borders of the image.
E) You ensure that only the same type of object is visible in each image.

removing black dots from image using OpenCV and Python

I am trying to compare two images and need to pre-process/clean one of them which is a scanned copy before comparing with a digital copy.
Scanned copy /
Digital copy
I ran this code on the scanned image and got an output which has numerous black dots. Not sure how to clean these up so that I can compare with the digital copy
img = cv2.multiply(img, 1.2)
kernel = np.ones((1, 1), np.uint8)
img = cv2.erode(img, kernel, iterations=1)
kernel1 = np.zeros( (9,9), np.float32)
kernel1[4,4] = 2.0
boxFilter = np.ones( (9,9), np.float32) / 81.0
kernel1 = kernel1 - boxFilter
img = cv2.filter2D(img, -1, kernel1)
below is the output I got
Try apply filter in frequency domain, your image after FFT will have regular bright dots, because your image noise. If you will remove these dots and make inverse FFT transform you will remove dots from your image. Check this examples please: example1 , example2 and example3.
Yes. #Andrey method is the right way of solving the problem.
I have tried removing the high frequency dots in the frequency domain and here is an example of how it will look like if done correctly
Original Image in grayscale.
After running FFT on the image
Removing all high frequency noise. Of course this is done manually by drawing a black circle around the noise source. You can design your program to detect local bright spot and remove them cleanly.
Here is the final result after inverse FFT of the above frequency image. Some what degraded due to the crude way of me removing the noise but it should give you a rough idea of how it can be done.
Only the area around the dots will be affected by this process, leaving all other pattern in their original form.

OpenCV (Python): Construct Rectangle from thresholded image

The image below shows an aerial photo of a house block (re-oriented with the longest side vertical), and the same image subjected to Adaptive Thresholding and Difference of Gaussians.
Images: Base; Adaptive Thresholding; Difference of Gaussians
The roof-print of the house is obvious (to the human eye) on the AdThresh image: it's a matter of connecting some obvious dots. In the sample image, finding the blue-bounded box below -
Image with desired rectangle marked in blue
I've had a crack at implementing HoughLinesP() and findContours(), but get nothing sensible (probably because there's some nuance that I'm missing). The python script-chunk that fails to find anything remotely like the blue box, is as follows:
import cv2
import numpy as np
from matplotlib import pyplot as plt
# read in full (RGBA) image - to get alpha layer to use as mask
img = cv2.imread('rotated_12.png', cv2.IMREAD_UNCHANGED)
grey = cv2.cvtColor(img,cv2.COLOR_BGR2GRAY)
# Otsu's thresholding after Gaussian filtering
blur_base = cv2.GaussianBlur(grey,(9,9),0)
blur_diff = cv2.GaussianBlur(grey,(15,15),0)
_,thresh1 = cv2.threshold(grey,0,255,cv2.THRESH_BINARY+cv2.THRESH_OTSU)
thresh = cv2.adaptiveThreshold(grey,255,cv2.ADAPTIVE_THRESH_GAUSSIAN_C, cv2.THRESH_BINARY,11,2)
DoG_01 = blur_base - blur_diff
edges_blur = cv2.Canny(blur_base,70,210)
# Find Contours
(ed, cnts,h) = cv2.findContours(grey, cv2.RETR_TREE, cv2.CHAIN_APPROX_SIMPLE)
cnts = sorted(cnts, key = cv2.contourArea, reverse = True)[:4]
for c in cnts:
approx = cv2.approxPolyDP(c, 0.1*cv2.arcLength(c, True), True)
cv2.drawContours(grey, [approx], -1, (0, 255, 0), 1)
# Hough Lines
minLineLength = 30
maxLineGap = 5
lines = cv2.HoughLinesP(edges_blur,1,np.pi/180,20,minLineLength,maxLineGap)
print "lines found:", len(lines)
for line in lines:
cv2.line(grey,(line[0][0], line[0][1]),(line[0][2],line[0][3]),(255,0,0),2)
# plot all the images
images = [img, thresh, DoG_01]
titles = ['Base','AdThresh','DoG01']
for i in xrange(len(images)):
plt.subplot(1,len(images),i+1),plt.imshow(images[i],'gray')
plt.title(titles[i]), plt.xticks([]), plt.yticks([])
plt.savefig('a_edgedetect_12.png')
cv2.destroyAllWindows()
I am trying to set things up without excessive parameterisation. I'm wary of 'tailoring' an algorithm for just this one image since this process will be run on hundreds of thousands of images (with roofs/rooves of different colours which may be less distinguishable from background). That said, I would love to see a solution that 'hit' the blue-box target - that way I could at the very least work out what I've done wrong.
If anyone has a quick-and-dirty way to do this sort of thing, it would be awesome to get a Python code snippet to work with.
The 'base' image ->
Base Image
You should apply the following:
1. Contrast Limited Adaptive Histogram Equalization-CLAHE and convert to gray-scale.
2. Gaussian Blur & Morphological transforms (dialation, erosion, etc) as mentioned by #bad_keypoints. This will help you get rid of the background noise. This is the most tricky step as the results will depend on the order in which you apply (first Gaussian Blur and then Morphological transforms or vice versa) and the window sizes you choose for this purpose.
3. Apply Adaptive thresholding
4. Apply Canny's Edge detection
5. Find contour having four corner points
As said earlier you need to tweak with input parameters of these functions and also need to validate these parameters with other images. As it might be possible that it will work for this case but not for other cases. Based on trial and error you need to fix the parameter values.

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